Desarrollo de un sistema de análisis por inyección en flujo (FIA) con detección amperométrica aplicando nanotubos de carbono en vinos blancos

Gabriel Alejandro  Moreno Toasa; Magdalena  Sailema-Ortiz; Yagos Arias  Carlos Jeanpier

doi:10.59169/pentaciencias.v5i7.925

Authors

Gabriel Alejandro Moreno Toasa Universidad Tecnica de Ambato https://orcid.org/0000-0002-1976-8699
Magdalena Sailema-Ortiz Universidad Técnica de Ambato https://orcid.org/0000-0002-6092-194X
Yagos Arias Carlos Jeanpier Universidad de las Fuerzas Armadas https://orcid.org/0009-0000-4978-2574

DOI:

https://doi.org/10.59169/pentaciencias.v5i7.925

Keywords:

FIA; Carbon nanotubes (MWCNTs); Wine; Spectrophotometry; Electroanalytical

Abstract

The research developed an analytical methodology for the analysis of total polyphenols in wines, using a flow injection analysis system (FIA) with amperometric detection, using an electrode modified with multilayer carbon nanotubes. For this purpose, the procedure for the elaboration of MWCNTs dispersions, using polyethylenimine as dispersing agent and its use for the modification of glassy carbon electrodes, was initially evaluated. The processing conditions were: 0.50 mg-mL-1 nanomaterial concentration, 0.25% (m/m) PEI, 2000 J of dispersion energy in PEI, centrifugation (2500 rpm; 20 min) and finally, redispersion in water applying 2000 J of energy. These electrodes show lower oxidation potentials, higher peak intensity and lower I/C ratios than the unmodified electrodes. Finally, this FIA methodology was developed incorporating the optimized electrode [GCE(PEI-Water/MWCNTs)], in order to detect and quantify total polyphenols, obtaining improvements in stability, reproducibility, greater sensitivity and possibility of selective detection of these compounds, in the analysis of white wines made with two types of grapes: Verdejo and Airén. This developed methodology could provide additional information from electrochemical indices, generating an alternative analysis to spectrophotometric studies.

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